66,842 research outputs found

    Geographical effects on epidemic spreading in scale-free networks

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    Many real networks are embedded in a metric space: the interactions among individuals depend on their spatial distances and usually take place among their nearest neighbors. In this paper, we introduce a modified susceptible-infected-susceptible (SIS) model to study geographical effects on the spread of diseases by assuming that the probability of a healthy individual infected by an infectious one is inversely proportional to the Euclidean distance between them. It is found that geography plays a more important role than hubs in disease spreading: the more geographically constrained the network is, the more highly the epidemic prevails.Comment: 4 pages, 5 figure

    Improved Performances Of Metal-Oxide-Nitride-Oxide-Silicon Memory With Hftion as Charge Trapping Layer

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    Molecular cloning and characterization of a cytoplasmic cyclophilin gene in sugarcane

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    Cyclophilins are ubiquitous proteins with an enzymatic activity of peptidyl-prolyl cis-trans isomerase (PPIase), which play important roles in a variety of stress responsiveness. In this study, we reported the cloning and characterization of a full-length cytoplasmic cyclophilin gene in sugarcane. Sequence analysis showed the cDNA of this gene (GenBank accession number:GQ246462), termed as Sc-CyP, was 904 bp long, including a 519 bp complete ORF, the 5’ UTR of 74 bp and 3’UTR of 311 bp, plus a typical AATAA motif and poly (A) tail. It encoded the 172 amino acid polypeptide with a molecular weight of 18.4 KD and the isoelectric point of 8.68. The Sc-CyP encoding protein had the conserved site Trp128 (W128) ubiquitious of all cyclophilins in eukaryotes and the KSGKPLH48-54 region specific to cytoplasmic cyclophilins in plants. SDS-PAGE analysis and PPIase assay revealed that the expression product, with PPIase activity, was a fusion protein with a molecular weight about 25 and 18.4 kD of Sc- CyP plus 7 kD of His • Tag peptides. In real-time qPCR analysis, the Sc-CyP gene showed induced expression under PEG, NaCl, SA and H2O2 stresses, indicating it a stress-related gene for drought and salt stress, signal transduction and disease resistance response in sugarcane.Key words: Sugarcane (Saccharum officinarum), cyclophilin, PPIase, real-time quantitative PCR

    Molecular cloning and expression analysis of a zeta-class glutathione S-transferase gene in sugarcane

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    Glutathione S-transferases (GSTs) play an important role in stress tolerance in plants. This is the first report of cloning and characterization of a zeta-class GST gene in sugarcane (GenBank Accession number: GQ246461). Sequence analysis showed that the cDNA sequence of Sc-GST gene was 829 bp, contained a 621 bp open reading frame (ORF), the 5’ untranslated region (UTR) of 65 bp and 3’UTR of 143 bp, plus the typical AATAA region and poly (A) tail. It encoded the 206 amino acid residues with a molecular mass of 23.1 KD and isoelectric point of 6.10. Protein domain prediction and multiple sequence alignment demonstrated that the conserved domain in Sc-GST at N-terminus was SSCXXRXRIA, while that at C-terminus was quebec platelet disorder (QPD), both of which were specific for zeta-type GST in eukaryotes. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and enzyme activity assay indicated that the prokaryotic expression product was a fusion protein with a molecular weight of about 30 KD, which also possessed GST enzyme activity. It was revealed in real-time quantitative polymerase chain reaction (qPCR) analysis that the Sc-GST gene had induced expression under H2O2 and Ustilago scitaminea stresses, while it was inhibited and then induced by salicylic acid (SA) stress, suggesting that it is a type of stress-tolerant gene playing a certain role in sugarcane resistance response.Key words: Saccharum officinarum, glutathione S-transferase, homology, prokaryotic expression, real-time quantitative PCR
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